Anti-phospho-PHDE1-A type I (Ser293)

Analysis Note

ControlUntreated and DCA-treated HEK293 cell lysates

Application

Immunocytochemistry Analysis: A 1:500 dilution of this antibody from a representative lot detected phospho-PDHE1-A type I (Ser293) in untreated and DCA-treated HEK293 cells.

Research Sub CategoryGlucose/Glycogen Metabolism

Research CategoryMetabolism

Detect phospho-PDHE1-A type I (Ser293) using this Anti-phospho-PDHE1-A type I (Ser293) Antibody validated for use in WB, IC & IP.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

In many organisms, the pyruvate dehydrogenase (PDH) complex catalyzes the overall, irreversible conversion of pyruvate to acetyl-CoA and CO2 in the aerobic pathway. This complex also serves as a key regulator for cardiac substrate selection. It contains multiple copies of three enzymatic components: pyruvate dehydrogenase (E1 or PDHE1-A type I), dihydrolipoamide acetyltransferase (E2), and lipoamide dehydrogenase (E3). PDH is regulated by both pyruvate dehydrogenase kinase (PDK)-mediated phosphorylation and feedback inhibition.

Immunogen

KLH-conjugated linear peptide corresponding to human PDHE1-A type I phosphorylated at Ser293.

Epitope: Phosphorylated Ser293

Physical form

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Quality

Evaluated by Western Blot in DCA untreated and treated HEK293 cell lysates.

Western Blot Analysis: A 1:10,000 dilution of this antibody detected phospho-PDHE1-A type I (Ser293) in untreated and DCA-treated HEK293 cell lysates.

Specificity

This antibody recognizes PDHE1-A type I phosphorylated at Ser293.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~43 kDa observed